ERGOVALINE MOVEMENT ACROSS CACO-2 CELLS

Permeability of Rice Cystatin across Caco-2 Cells

The present work aimed at recovering, purifying, and testing rice cystatin or oryzacystatin I (OCI) bioavailability, to provide scientific evidence that rice cystatin can be used as a functional food ingredient. OCI was extracted from rice bran using 25 mM sodium phosphate buffer containing 0.15 M NaCl at pH 7.0. The resulting homogenate was heat treated, cooled and precipitated with ammonium s...

Transport Across Caco-2 Monolayer: Biological, Pharmaceutical and Analytical Considerations

Transport of hop aroma compounds across Caco-2 monolayers.

Although being reported and used as a sedative remedy for several years, the bioactive principle of hop preparations is still not decisively clarified. Understanding absorption and transformation processes of potential physiologically active constituents is essential to evaluate the likeliness of biological effects on humans. Therefore, single hop aroma compounds as well as digestive transforma...

DNA-Fragments Are Transcytosed across CaCo-2 Cells by Adsorptive Endocytosis and Vesicular Mediated Transport

Dietary DNA is degraded into shorter DNA-fragments and single nucleosides in the gastrointestinal tract. Dietary DNA is mainly taken up as single nucleosides and bases, but even dietary DNA-fragments of up to a few hundred bp are able to cross the intestinal barrier and enter the blood stream. The molecular mechanisms behind transport of DNA-fragments across the intestine and the effects of thi...

Pseudomonas fluorescens alters epithelial permeability and translocates across Caco-2/TC7 intestinal cells

BACKGROUND Pseudomonas fluorescens has long been considered as a psychrotrophic microorganism. Recently, we have shown that clinical strains of P. fluorescens (biovar 1) are able to adapt at a growth temperature of 37°C or above and induce a specific inflammatory response. Interestingly, a highly specific antigen of P. fluorescens, I2, is detected in the serum of patients with Crohn's disease b...